Table 2.

Summary of serological methods for SeACoV antibody detection.

Method	Target protein	Principle	Reference
IFA	Live virus	To examine SeACoV antibody from pig sera in SeACoV-infected cells	Pan et al. (2017)
ELISA	S1 domain	Antibody assay based on the S1 domain of the S protein using a luciferase immunoprecipitation system	Zhou et al. (2018b)
	Virus particles	Purified virus particles as antigen to detect SeACoV antibodies in the serum from the infected mice	Yang et al. (2019b)
	Not defined	Serological investigation of recent SADS cases implied that SADS-CoV, rather than PEDV, may directly contribute to the diarrhea	Zhou et al. (2019a)