Figure 2.

NCoR1^ΔIEC mice show increased epithelial permeability after DSS treatment and altered proliferative cells. (A) FITC-d permeability analysis. NCoR1^F/F and NCoR1^ΔIEC mice were treated with water or DSS for 3 or 5 days, respectively. On the last day, each mouse was administered 20 mg of FITC-d through oral gavage. After 4 hours, blood samples were collected for serum, and FITC-d concentrations were measured and calculated from a FITC-d standard curve. Data are described as FITC concentration (n = 6). (B) BrdU incorporation analysis. Mice were treated with BrdU at 100 mg/kg by intraperitoneal injection on the last day of DSS exposure. Slides were examined under an upright Imager A2 microscope (Zeiss), at least 10 photographs of different areas were taken of each slide. Scale bar: 40 μm. BrdU+ cells were enumerated and described as BrdU+ cells per villous (n = 5). (C) On day 3 after DSS exposure, colon samples were dissected for immunostaining of Ki67. Photographs were taken under a Leica TCS SP5 X confocal microscope and LAS AF imaging software. Scale bar: 50 μm. Ki67+ cells were counted and described as average Ki67+ cells per field (n = 6). DAPI, 4′,6-diamidino-2-phenylindole. ∗P < .05, ∗∗P < .01.