Figure 5.

Synergetic role of butyrate, TNFα, and NCoR1 deletion in inducing crypt cell apoptosis. (A) Cells were treated with different concentrations of butyrate in the presence or absence of TNFα (20 ng/mL). Twenty-four hours after treatment, cells were collected and whole-cell extracts were prepared for Western blot analysis. (B) Cells were treated with different concentrations of trichostatin (TSA) in the presence or absence of TNFα (20 ng/mL) for 24 hours and followed by the preparation of whole-cell extracts that were used in Western blot analysis. (C) Cells were treated with butyrate (5 mmol/L) in the presence or absence of anacardic acid (ANA, 10 μmol/L, MedChemExpress), a histone acetyltransferase inhibitor. After 24 hours, whole-cell extracts were prepared for electrophoresis and Western blot. The band densities of targets and tubulin were quantified using Image Lab 5.2.1 software, and the results were described as the ratio of band density of targets over tubulin. BUT, butyrate; C-Casp, cleaved caspase.