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. 2020 May 6;2020:8361798. doi: 10.1155/2020/8361798

Table 2.

Characteristics of included studies.

Studies Study design Fusion type Conc of PLT in PRP Preparation of PRP Material used Sex (m/f) Follow-up time (months) Evaluation
1. Kubota et al. [15] Retrospective case series Lumbar PLF 8.7 400 mL of peripheral blood centrifuged. Buffy coat isolated. The second centrifugation done. 22 mL mixed with calcium chloride. Local bone, CA, PRP (n = 11)
Local bone CA (n = 9)
10/10 24 CT

2. Kubota et al. [9] Prospective Lumbar PLF 7.7 400 mL of peripheral blood centrifuged. Buffy-coat isolated. The second centrifugation done. 22 mL mixed with calcium chloride. ABG lamina, PRP (n = 25)
ABG lamina (n = 25)
29/21 24 Radiograph, CT

3.Tarantino et al. [10] Prospective cohort Lumbar PLF Not measured Venous blood centrifuged at 3100 rpm for 8 minutes. Buff coat removed. Platelets were suspended in plasma while shaking the tubes and were ready for use. Heterologous bone, PRP (n = 20)
Heterologous bone (n = 20)
8/12 6 CT

4. Sys et al. [16] Prospective Lumbar IF Not measured 54 mL of peripheral blood put in SymphonyTM Platelet Concentration System (DePuy, Johnson & Johnson). CA, ABG iliac, PRP (n = 19)
CA, ABG iliac (n = 19)
24/14 12 Radiograph, CT

5. Land et al. [17] Retrospective Thoracic or lumbar IF 5 16 mL of peripheral blood in REGEN-THT_ (thrombocyte harvesting tube) followed by the addition of Ca gluconate and ethanol. ABG local bone, PRP (n = 14)
ABG local bone (n = 14)
9/5 6 Radiograph CT

6. Tsai et al. [18] Prospective Lumbar PLF Not measured 10 mL of FFP mixed with calcium chloride. Mixture shaken for 30 minutes and ready for use. ABG lamina PRP (n = 33)
ABG lamina (n = 34)
17/50 24 Radiograph, CT

7. Hartmann et al. [11] Prospective Lumbar or thoracic PLF Not measured 110 mL PVB in Gravitational Platelet Separation (GPS™) System mixed with thrombin. CA, ABG fracture, PRP (n = 15)
CA, ABG fracture (n = 20)
23/12 8 CT

8. Feiz-Erfan et al. [12] Prospective Cervical IF Not measured Anticoagulated blood put in Symphony (DePuy, Johnson & Johnson). Cortical allograft bone, PRP (n = 42)
Cortical allograft bone alone (n = 39)
21/29 24 Radiograph

9. Jenis et al. [14] Prospective Lumbar PLF Not measured 450 mL PVB in centrifuge. Buffy coat mixed with thrombin. Allograft bone, PRP(n = 15)
ABG iliac bone (n = 22)
24/13 24 Radiographic CT

10. Carreon et al. [13] Retrospective cohort study Lumbar PLF Not measured 500 mL PVB, in a centrifuge. A buffy coat mixed with calcium chloride. ABG iliac, PRP (n = 76)
ABG iliac alone (n = 76)
40/36 24 Radiographic CT

11. Castro et al. [19] Retrospective TLIF 3.5 1 unit of PVB in centrifuge. A buffy coat mixed thrombin AGF, iliac bone (n = 22) iliac bone (n = 62) 27/57 41 Radiograph

12. Hee et al. [20] Prospective Lumbar PLF 4.89 450 cc of WB in centrifuge buffy coat mixed with thrombin CA, ABG iliac (n = 23)
ABG iliac (n = 111)
50/84 24 Radiograph

13. Weiner and Walker [21] Retrospective Lumbar PLF Not measured From 1 unit of arterial blood, buffy coat collected and put in ultraconcentrator. Thrombin was added and the PRP was ready for use. ABG iliac PRP(n = 32)
ABG iliac (n = 27)
24/35 12 Radiograph

14. Imagama et al. [2] Prospective Lumbar PLF 7.74 400 mL of WB put in centrifuge for 15 minutes, buffy coat collected. Buffy coat put in centrifuge again for 15 minutes followed by the addition of thrombin. ABG local bone and PRP(n = 29)
ABG local bone (n = 29)
11/18 120 CT

CA = cage; PRP = platelet-rich plasma; ABG = autogenous bone graft; CT = computed tomography; IF = interbody fusion; PLF = posterolateral fusion; TLIF = transforaminal lumbar interbody fusion; PVB = peripheral venous blood; WB = whole blood; PLT = platelet; Conc = concentration.