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. 2020 May 15;18:76. doi: 10.1186/s12951-020-00636-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Differentiation status of the chicken embryo muscle control cells and treated with graphene oxide nanofilm (nGO), l-glutamine (L-Glu), and on nGO with L-Glu supplementation (nGOxL-Glu). a Confocal microscopy visualisation images (×40 magnification): grey = Nomarski interference contrast, blue = 4′,6-diamidino-2-phenylindole (DAPI) nuclei staining, red = actin filaments with phalloidin-Atto 633 staining; b quantitative analysis of the fusion index of the differentiating cells. The error bars represent standard error of mean (n = 8 per group). Different letters (a–b) above the columns indicate statistically significant differences (p ≤ 0.05) analysed by Tukey’s HSD test