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. 2020 May 15;16:71. doi: 10.1186/s13007-020-00614-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A schematic plan describing the multiplex RNAscope-ISH assay to visualize the spatio-temoral expression of more than one target RNA species in a tissues section. Fixed plant tissue section with preserved RNA in each cell (A). RNA species can be targeted or non-targeted. Binding of target ZZ probe pair (B), PreAMPs (C), AMPs and fluorescence probes (D) in a cell containing the target RNA species. Inability of ZZ probe pair, PreAMP, AMP and fluorophore binding (a, b, c) in cells containing non-targeted RNA species only. Visualization of probe fluorescence in the tissue section in spatio-temporal manner for targeted RNA species (E)