Fig. 3.

Levels of intracellular rhodamine 123 (R123) in CEM and CEM-VCR R cell lines in the presence and absence of cyclosporine A (CSA). a Parental (CEM) and VCR-resistant cells (CEM-VCR R) were incubated with R123 for 20 min before being washed with PBS and lysed with 1% Triton-X. b CEM-VCR cells were incubated with R123 in the presence or absence of 20 µM Cyclosporin A (CSA), a specific P-gp inhibitor, for 20 min before undergoing subsequent washing and lysis. Intracellular fluorescence was subsequently measured using a FLUOstar Optima Microplate Reader using an excitation wavelength of 485 nm, and emission wavelength of 530 nm. Mean ± SD, n = 3. ***P < 0.001, ****P < 0.0001