Table 3.

Functional annotation analysis of predicted gene targets of identified candidate miRNAs

Candidate miRNA	Number of annotation clusters with enrichment score > 1.3 for predicted gene targets	Annotation cluster	Gene count*	Enrichment score	p value	FDR
miR-146b-5p	34	Innate immune response	24	13.8	6.1E−13	3.0E−10
		Cellular response to cytokine stimulus	33	11.72	1.9E−15	3.21E−12
		Defence response to bacterium	25	9.43	2.4E−12	4.03E−09
		Interleukin-1 receptor binding	7	6.35	3.66E−10	4.83E−07
		Regulation of interleukin-6 production	13	4	7.4E−11	1.26E−07
		Regulation of interleukin-12 production	7	3.87	3.89E−06	0.006623
		Lipopolysaccharide-mediated signalling pathway	8	3.82	1.50E−07	2.55E−04
		Regulation of cytokine secretion	10	3.68	4.44E−06	0.007556
miR-15b-5p	31	Apoptotic process	91	6.08	2.2E−9	4.04E−06
		Regulation of protein metabolic process	119	5.71	9.9E−10	1.80E−06
		Regulation of cell cycle	36	2.55	3.2E−6	0.005875

Functional annotation clustering analysis of target mRNA gene lists of candidate miRNAs using DAVID. Top annotation clusters are listed

*Number of genes from the target mRNA gene list submitted for each miRNA (158 genes for miR-146b-5p and 526 genes for miR-15b-5p) that are involved in the annotation cluster