Fig. 8.

Cell types and gene expression in the outflow pathways of the mouse. (A) A t-distributed stochastic neighbor embedding (tSNE) plot showing 20 cell types derived from TM and associated structure of mouse. (B) Transcriptional correspondence between human and mouse cell types shown as in Fig. 6B. Fibro, fibroblast; K-Epi, corneal epithelium; Melano, melanocyte; MØ, macrophage; SchMy, myelinating Schwann cell; SchNmy, nonmyelinating Schwann cell; Schwal, Schwalbe line; VEndo, vascular endothelium. (C) Violin plot showing examples of genes selectively expressed by each cell type in mouse. K-Endo, corneal endothelium; NPCE, nonpigmented ciliary epithelium; PE, pigmented epithelium; Schwn, Schwann cell. (D and E) Pdpn (red) is present in multiple cell types, including pigmented and nonpigmented epithelium of the iris and ciliary body (CB) as well as a subset of TM cells, K-Endo, and K-Epi, whereas Chil1 (green; ortholog to CHI3L1 in humans) stains a different subset of TM cells and to a lesser extent, cells within the CB. (E) Higher magnification of the boxed area in D. (F and G) Immunostaining against Postn (green), a secreted protein, highlights SC and JCT cells. Pecam1 (red) highlights SC as well as vascular endothelial clusters. AF, autofluorescence. DAPI, 4′,6-diamidino-2-phenylindole (nuclear stain). (Scale bars: 50 µm.)