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. Author manuscript; available in PMC: 2020 May 16.
Published in final edited form as: J Leukoc Biol. 2020 Feb 4;107(4):663–671. doi: 10.1002/JLB.3A0120-379RR

FIGURE 9. IL-33 and IL-12 regulate TNF release via ADAM17.

FIGURE 9

Enriched human NK cells were pretreated with 0.1% DMSO, R-1503 (0.25 μM) or TAPI-1 (8 μM) and then stimulated in the presence of cytokines (doses listed in ng/mL) for 16 h. (A) Secreted TNF expression was measured by ELISA where each dot represents the mean of 3 biological replicates of an independent subject (n = 6). (B) Cell-associated TNF was measured by flow cytometry where each dot represents the mean of 2 biological replicates of an independent subject (n = 4). Shaded bars reflect administration of >1 cytokine. Multiple comparisons of ordinary 1-way ANOVA were used to compare columns with P < 0.1 shown