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. 2020 Apr 24;117(19):10368–10377. doi: 10.1073/pnas.2001812117

Fig. 1.

Fig. 1.

The chromosomal distribution of rDNA in nontransformed human cell lines. (A) Schematic showing the FISH probes used in this study. (B) FISH performed on metaphase spreads prepared from hTert-RPE1 (Left) and CCD-1079Sk (Right) cells. rDNA and DJ probes are in red and green, respectively, and chromosomes were stained with DAPI. (C) FISH performed on metaphase spreads prepared from hTert-RPE1 with rDNA (red) and α-satellite probes indicated. Note that α-satellite probes from HSA13 and HSA21 cross-react, as do HSA14 and HSA22. The HSA15 probe is unique to that chromosome. (D) CCD-1079Sk metaphase spreads probed with rDNA (red), HSA14 and HSA22 α-satellite (green), and HSA15 (red and green, resulting in yellow). Note that HSA13 and HSA21 are distinguished by size. (E) NOR ideograms for hTert-RPE1, 1BR3, and CCD-1079Sk cells. The relative rDNA contents (red) for each NOR are shown below a DJ (green). Chromosomal identities are shown on top, and UBF loading status is indicated under each NOR ideogram.