Fig. 3.

DB28 down-regulates MR1 from the cell surface of primary cells. CD2-depleted PBMC were incubated overnight with MG (50 μM), 5-OP-RU (1 μg/mL) with or without DB28 (20 μg/mL), and MR1 expression on the surface of gated live B cells (A and B) or monocytes (C and D) was determined by flow cytometry. Cumulative data of geometrical mean fluorescence intensity of four different blood donors (A and C); representative FACS histograms (B and D). The background staining of isotype control is shown in black.