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DB28 down-regulates GPI-linked MR1 molecules, but not K43A mutants. THP1 MR1 KO cells overexpressing WT MR1 molecules, K43A mutants (A), or GPI-linked MR1 (B) were pulsed for 5 h with the indicated ligands (MG 50 μM, Ac-6-FP 1 μg/mL, 5-OP-RU 5 μg/mL, DB28 20 μg/mL) before staining with anti-MR1 (26.5) antibody. Geo MFI ± SD of technical duplicates are plotted in each graph. Data representative of three experimental replicates.
