Skip to main content
. Author manuscript; available in PMC: 2020 May 16.
Published in final edited form as: Nature. 2019 May 13;570(7759):77–82. doi: 10.1038/s41586-019-1184-5

Extended Data Figure 3: Experimental overview.

Extended Data Figure 3:

a. Schematic of platform used for generation of single cell RNA-seq libraries and corresponding target site amplicon libraries, adapted from Adamson et al., 2016 (Ref 18). The barcoded and amplified cDNA library is split into two fractions prior to shearing: one fraction is used to generate a global transcription profile and the other is used to specifically amplify the target site.

b. Summary table of lineage traced embryos detailing the type of guides used, the sampling proportion, and sequencing results. Embryo 4 was omitted from further analysis due to the absence of cells identified as primitive heart tube.