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. 2020 Apr 8;48(9):5157–5168. doi: 10.1093/nar/gkaa227

Figure 1.

Figure 1.

The VCR of METTL16 enhances U6 snRNA methylation. (A) Schematic diagrams of human METTL16 and its variants. METTL16_FL: full-length METTL16, MTD: methyltransferase domain, and VCR: vertebrate conserved region. VCR consists of VCR1 (pink) and VCR2 (green) connected by a linker. VCR_ΔL is VCR with the deletion of the linker and was used for crystallization and structural determination. (B) Methylation of U6 snRNA by METTL16_FL and MTD under standard conditions (1 μM of U6 snRNA). (C) Steady-state kinetics of m6A modification of U6 snRNA by METTL16_FL and MTD (Supplementary Figure S1A). (D) Gel-shift of U6 snRNA by METTL16_FL (0–0.125 μM) and MTD (0–8 μM) (Supplementary Figure S1B). (E) Methylation of U6 snRNA by METTL16_FL and METTL16_ΔL under standard conditions, as in (B). (F) A stereo view of the structure of VCR_ΔL. Amino acid residues 310–381 and 513–562 are modeled in the structure. VCR1 and VCR2 are colored purple and green, respectively. Bars in the graphs in (B)–(E) are standard deviations (SD) of three independent experiments.