Figure 5.
A model of cooperative U6 snRNA methylation by MTD and VCR. (A) Secondary structures of U6 snRNA variants used for assays in (B) and (C). (B) Steady-state kinetics of m6A modification of TSmt by METTL16_FL and MTD. (C) Steady-state kinetics of m6A modification of ΔTS3 by METTL16_FL and MTD. Bars in the graphs in (B) and (C) are standard deviations (SD) of three independent experiments. (D) A model of the cooperative methylation of U6 snRNA by the MTD and VCR of METTL16. VCR binding to ISL would promote the transition of the structural configurations of telestem-bulge-ISL in U6 snRNA to the bent form and enable the transition structure to be formed in the junction between the telestem and ISL for productive catalysis by the MTD.