Table 1.
Data collection and refinement statistics
| SeMet-METTL16_VCRΔL | Native-METTL16_VCRΔL | |
|---|---|---|
| Data collection | ||
| Space group | R32 | R32 |
| Cell dimensions | ||
| a, b, c (Å) | 139.59 | 139.61 |
| 139.59 | 139.61 | |
| 53.31 | 53.13 | |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 |
| Wavelength (Å) | 0.97800 | 0.98000 |
| Resolution (Å)* | 50–3.0 (3.11–3.00) | 50–2.8 (2.89–2.79) |
| R sym* | 0.332 (2.973) | 0.305 (4.265) |
| I/σI * | 14.8 (1.9) | 14.9 (1.1) |
| CC1/2* | 0.998 (0.684) | 0.999 (0.523) |
| Completeness (%)* | 100.0 (100.0) | 99.8 (98.4) |
| Redundancy* | 40.6 (42.2) | 40.9 (41.6) |
| Phasing | ||
| Se sites | 3 | |
| Mean FOM | 0.378 | |
| Refinement | ||
| Resolution (Å) | 50-2.8 | |
| No. reflections | 5013 | |
| R work/Rfree (%) | 23.36/26.31 | |
| No. atoms | ||
| Protein | 122 | |
| B-factors (Å2) | ||
| Protein | 85.4 | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.012 | |
| Bond angles (°) | 1.44 | |
| Ramachandran | ||
| Preferred (%) | 92.4 | |
| Allowed (%) | 7.6 | |
| Outliers (%) | 0 |
*Values in parentheses are for the highest-resolution shell.