Table 1.

Comparison of fitted conformation selection parameters to independently determined values

Parameter	Fit value	Independent estimate	Comment
ΔGc, kcal mol−1	+3.3 ± 0.2, +3.1 ± 0.1	+4.0 ± 0.6	First steps in G4 unfolding determined by thermal denaturation experiments (22)
nPOT1/DNA	2.2 ± 0.1	2.0	Determined by AUC (Figure 4)