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Modification activity of Ct C/D RNPs assembled with human C/D-like snoRNAs. (A) Secondary structural models of human C/D-like snoRNAs. The predicted substrates on rRNAs and snRNAs (a series) and perfectly paired substrates (b series) are shown. (B) Activity of C/D RNPs assembled with human C/D-like snoRNA (2 μM), Snu13 (6 μM), Nop56/Fib(2 μM) and Nop58/Fib (2 μM). Unmethylated and pre-methylated (-m) substrates were modified for 20 min at 50°C.
