Table 2.
Expression levels of cognate miRNAs and stem loops to the five conserved binding sites shared by AGO phosphorylation cycle mRNAs. The miRNA expression levels in the HCT116 cells were computed, as described in Materials and Methods, based on the miRNA-seq dataset in Kim et al. (31). The ranks are also listed for the miRNA ranked among the top-100 highest expressed miRNAs. The stem loop expression levels were collected from the miRbase website (www.mirbase.org). ND: Not Detected
| Cognate miRNAs | Cognate stem loops | ||||
|---|---|---|---|---|---|
| Sites | Name | Expression level (RPM) | Expression level ranking | Name | Expression level (RPM) per miRBase |
| miR-17-5p/20-5p/93-5p/106- 5p/519-3p | miR-17-5p | 874.28 | 78 | miR-17 | 9.52e3 |
| miR-20a-5p | 554.38 | 98 | miR20a | 7.48e3 | |
| miR-20b-5p | 0.188 | miR-20b | 545 | ||
| miR-93-5p | 4017.82 | 42 | miR-93 | 2e3 | |
| miR-106a-5p | ND | miR-106a | 7.94e3 | ||
| miR-106b-5p | 974.99 | 72 | miR-106b | 1.84e3 | |
| miR-519d-3p | ND | miR-519d | 3.23 | ||
| miR-30-5p | miR-30a-5p | 292.2 | miR-30a | 1.51e4 | |
| miR-30b-5p | 1057.2 | miR-30b | 2.25e3 | ||
| miR-30c-5p | 3217 | 45 | miR-30c-1 | 3.21e3 | |
| miR-30c-2 | 3.22e3 | ||||
| miR-30d-5p | 16185.82 | 17 | miR-30d | 1.17e4 | |
| miR-30e-5p | 15738.3 | 18 | miR-30e | 1.07e4 | |
| miR-103-3p/107 | miR-103a-3p | 5902.59 | 30 | miR-103a-1 | 1.24e4 |
| miR-103a-2 | 1.24e4 | ||||
| miR-107 | 748.65 | 87 | miR-107 | 1.19e4 | |
| miR-137 | miR-137 | ND | miR-137 | 246 | |
| miR-302-3p/372-3p/373-3p/520-3p | miR-302a-3p | 0.19 | miR-302a | 42.9 | |
| miR-302b-3p | ND | miR-302b | 0 | ||
| miR-302c-3p | ND | miR-302c | 0 | ||
| miR-302d-3p | 0.87 | miR-302d | 10.3 | ||
| miR-302e | ND | miR-302e | 0 | ||
| miR-372-3p | 0.56 | miR-372 | 91.9 | ||
| miR-373-3p | 0.68 | miR-373 | 2.22 | ||
| miR-520a-3p | ND | miR-520a | 4.76 | ||
| miR-520b-3p | ND | miR-520b | 9.8 | ||
| miR-520c-3p | ND | miR-520c | 6.02 | ||
| miR-520d-3p | ND | miR-520d | 0 | ||
| miR-520e-3p | ND | miR-520e | 33.3 | ||