Figure 1.

Identification of residues critical for DNA binding by MipZ. (A) Surface representation of the MipZ dimer structure (PDB ID: 2XJ9) with the 51 amino acids selected for alanine-scanning mutagenesis marked in purple. The two subunits are depicted in green and blue, respectively. (B) Cartoon representation of the MipZ dimer structure with nine candidate amino acids possibly involved in DNA binding shown in purple. A surface representation of the structure is shown in the background. For clarity, the ATP molecules and Mg²⁺ ions sandwiched between the two subunits are not shown.