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. 2020 Mar 30;48(9):4769–4779. doi: 10.1093/nar/gkaa192

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Global distribution of chromosomal MipZ binding sites. (A) ChIP-seq analysis of the distribution of different MipZ variants across the C. crescentus chromosome. Cells producing a wild-type (BH64), monomeric (K13A; BH100) or dimeric (D42A; BH99) MipZ-eYFP fusion in place of the native protein were fixed with formaldehyde and subjected to ChIP-seq analysis with an anti-GFP antibody. A representative 50 kb window of the chromosome is shown for visualization. Data were normalized using wild-type strain NA1000 as a reference. (B) Biolayer interferometric analysis of the interaction of MipZ with DNA of different GC content. Biotinylated dsDNA oligonucleotides with GC contents of 17% (ATrich-biotin/ATrich-rev), 56% (GC56-biotin/GC56-rev) or 79% (GCrich-biotin/GCrich-rev) were immobilized on biosensors and probed with wild-type MipZ (4 μM) in the presence of ATPγS (1 mM).