Figure 6.

Metabolites of SSOs ORN1, ORN_st, ORN₈₄ and ORN_ch from tissues after administration to Emi/wt mice. (A) Animals received four injections at 50 mg/kg in 0.9% saline over 2 weeks and were sacrificed 24 h after the last dose. Oligonucleotides were extracted from the tissue matrix using the Phenomenex Clarity OTX kit. Tissues from mice treated with ORN1_ch were subjected to a proteinase K digestion before processing. Detected oligonucleotide species are marked in red, next to their associated masses determined by LC–MS (see also Supplementary Figures S9–S12). (B) Calculated molecular weights of parent SSOs and their inferred metabolites from LC–MS data shown in a) (C17 is the stearoyl chain; chol is the thiocholesteryl group).