Figure 7.

Treatment of Emi/wt mice with SSOs modulates splicing in selected tissues. Animals received four injections at 50 mg/kg in 0.9% saline over 2 weeks and were sacrificed 24 h after the last injection. Levels of correct FECH transcript were assessed by SYBR green RT-qPCR and normalized to GAPDH and to the saline group. n = 3 animals for the saline group; n = 4 animals for oligonucleotide treated groups; statistical analysis (t tests) was conducted with GraphPad Prism software: *P < 0.05; **P < 0.01 (whiskers indicate lowest and highest values; middle line represents the median).