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. 2020 Apr 21;48(9):5183–5195. doi: 10.1093/nar/gkaa239

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Survival cocktail and conditions for clonal derivation of iPSCs after large-scale genome engineering. (A) Human iPSC transfection timeline and survival cocktail conditions. (B) Eighteen-hour single cell direct NGS analysis of dABE targeting LINE-1. CS and PK indicate the two researchers who conducted the experiments. The red line indicates the median and the blue line the mean. (C) Live cell colony analysis of surviving iPSCs at day 11 post-transfection.