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. 2020 Mar 30;48(9):4946–4959. doi: 10.1093/nar/gkaa189

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — The assembly of hcLRS into the MSC complex. The gel filtration result of hcLRS mixed with EPRS (A), or MRS-AIMP3 (B), or IRS (C), the peak of each component are indicated by the elution volume; The in vitro assembly of human MSC complex by all 11 protein components (D) or lacking IRS (E) or lacking LRS (F); Figure G shows the gel filtration result of the hcLRS_d106 and IRS mixture; Figure H shows the in vitro assembly of human MSC complex using hcLRS_d106 instead of hcLRS.