Figure 1.

Acr candidates selected for biochemical testing. Ten Acr candidates were selected from manual inspection for further biochemical testing (blue fill). Each candidate is shown in its genomic context with its assigned rank from AcRanker noted in red. Homologous proteins share the same color border (green, blue). Homologs of AcrIIA3 (orange border) and AcrIIA1 (red border) are indicated. While testing the ML candidates, ML3 (yellow fill) was identified as a specific inhibitor of LmoCas9 (25).