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Effect of Pin1-Ala mutants on Pho1 expression. (A) S. pombe rpb1-CTD-S7A strains bearing the indicated pin1 alleles were spot-tested for growth on YES agar at the indicated temperatures. (B) S. pombe strains bearing the indicated pin1 alleles in the context of the wild-type or S7A Pol2 CTD backgrounds were grown in liquid culture at 30°C and assayed for acid phosphatase activity.
