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. 2020 Apr 13;48(9):4627–4642. doi: 10.1093/nar/gkaa186

Figure 4.

Figure 4.

Taq polymerase stop assay on LTR-II+III+IV and control hTel templates. (A) LTR-II+III+IV and hTel templates were amplified by Taq polymerase at 42°C in the absence (lanes 8 and 15) and presence of 1 × 10−1 M KCl, alone (lanes 9 and 16) or with 4 × 10−7 M of NDI-PNA 5 (lanes 10 and 17), NDI-PNA 4 (lanes 11 and 18), NDI-PNA 3 (lanes 12 and 19), NDI-PNA 1 (lanes 13 and 20), or NDI 6 (lanes 14 and 21). A template (non-G4 cnt) made of a scrambled sequence unable to fold into G4 was also used as negative control (lanes 1–7). Lane P: unreacted labeled primer. Lane M: ladder of markers obtained by the Maxam and Gilbert sequencing carried out on the amplified strand complementary to the template strand. Vertical bars indicate G4-specific Taq polymerase stop sites. The three gel portions derive from a single gel run. (B) Quantification of lanes 8–14 shown in panel A. Quantification of stop bands corresponding to hTel G4 and of the full-length amplification product (FL) is shown. (C) Quantification of lanes 15–21 shown in panel A. Quantification of stop bands corresponding to LTR-II, LTR-III, LTR-IV G4s and of the full-length amplification product (FL) is shown.