Skip to main content
. 2020 Apr 13;48(9):4627–4642. doi: 10.1093/nar/gkaa186

Figure 5.

Figure 5.

Taq polymerase stop assay on LTR-III+IV and control hTel templates. (A) LTR-III+IV and hTel templates were amplified by Taq polymerase at 42°C in the absence (lanes 8 and 15) and presence of 1 × 10−1 M KCl, alone (lanes 9 and 16) or with increasing amounts (1 × 10−7, 2 × 10−7 and 4 × 10−7 M) of NDI-PNA 6 (lanes 10–12 and 17–19), 4 × 10−7 M of PNA 7 (lanes 13 and 20) or 4 × 10−7 M of NDI 6 (lanes 14 and 21). A template (non-G4 cnt) made of a scrambled sequence unable to fold into G4 was also used as negative control (lanes 1–7). Lane P: unreacted labeled primer. Lane M: ladder of markers obtained by the Maxam and Gilbert sequencing carried out on the amplified strand complementary to the template strand. Vertical bars indicate G4-specific Taq polymerase stop sites. * indicates the stop site corresponding to the binding of the PNA to its complementary sequence on the LTR-III+IV template. (B) Quantification of lanes 8–14 shown in panel A. Quantification of stop bands corresponding to hTel G4 and of the full-length amplification product (FL) is shown. (C) Quantification of lanes 15–21 shown in panel A. Quantification of stop bands corresponding to LTR-III, LTR-IV G4s and of the full-length amplification product (FL) is shown.