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. 2020 Apr 13;48(9):4627–4642. doi: 10.1093/nar/gkaa186

Figure 7.

Figure 7.

Competition Taq polymerase stop assay. (A) LTR-III+IV template was amplified by Taq polymerase at 42°C in the presence of 1 × 10−1 M KCl combined with a constant amount (4 × 10−7 M) of NDI-PNA 6 and increasing concentrations (1–8-fold) of competitor, LTR-III+IV (lanes 5–8) or hTel (lanes 9–12). Lanes without competitor (lanes 1–4) were used as internal controls, in the absence (lane 1) and presence of 1 × 10−1 M KCl, alone (lane 2), or combined with 4 × 10−7 M of NDI-PNA 6 (lane 3) or NDI 6 (lane 4). Lane P: unreacted labeled primer. Lane M: ladder of markers obtained by the Maxam and Gilbert sequencing carried out on the amplified strand complementary to the template strand. Vertical bars indicate G4-specific Taq polymerase stop sites. (B) Quantification of lanes 1–12 shown in panel A. Quantification of stop bands corresponding to LTR-III, LTR-IV G4s and of the full-length amplification product (FL) is shown.