Figure 2.

Plasmid expression of promoter library and RBS variants. Built by introducing ‘N’ variability surrounding the −35 and −10 boxes of Trc in the context of pBWB162, our promoter library (A) shows 73-fold expression range for LB and 60-fold expression range for M9 with PCA. (B) Utilizing the BCD design to create RBS variants, with the promoter held constant (Trc), we observe expression variance related to ribosomal binding strength. All experiments were performed as biological triplicate on three separate days (nine replicates), except LB expression of D12G6, which only had eight replicates. Error bars represent SEM. SD = Shine-Delgarno.