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. 2020 Feb 25;48(9):e49. doi: 10.1093/nar/gkaa133

Figure 3.

Figure 3.

(A) Field-swept electron spin echo spectrum of Cu2+-DPA–DNA (n = 11) at Q-band frequency. The lines show the different magnetic fields at which DEER was performed. (B) Background subtracted time domain DEER signals at the different magnetic fields. The y-axis has been offset for ease of visualization. The data show minimal difference in dipolar frequency at the different fields. (C) Background subtracted time domain data at g (dashed, 11220 G) and g (solid, 11820 G) regions. These two regions also do not show any distinct difference in modulation frequency, suggesting Cu2+–DPA shows minimal orientational effects. (D) Distance distribution obtained via Tikhonov regularization, for both g (dashed, 11220 G) and g (solid, 11820 G) regions. The similarity in the distributions further confirms that Cu2+–DPA is not orientation selective for non-complexed DNA.