Table 2.
Primers used for qPCR analyses of immune cytokines, tight junction protein, and MUC 2
| Target | Amplicon size, bp | Primer sequence (5′ to 3′) | Reference1 |
|---|---|---|---|
| GAPDH | 142 | F: GTGAACGGATTTGGCCGC | NM_001206359.1 |
| R: AAGGGGTCATTGATGGCGAC | |||
| IL-1β | 91 | F: TGGCTAACTACGGTGACAACA | NM_214055 |
| R: CCAAGGTCCAGGTTTTGGGT | |||
| IL-4 | 243 | F: TCCACGGACACAAGTGCGAC | NM_214123 |
| R: TGTTTGCCATGCTGCTCAGG | |||
| IL-6 | 151 | F: AAGGTGATGCCACCTCAGAC | M86722 |
| R: TCTGCCAGTACCTCCTTGCT | |||
| IL-8 | 126 | F: AGAGGTCTGCCTGGACCCCA | NM_213867 |
| R: GGGAGCCACGGAGAATGGGT | |||
| IL-10 | 220 | F: CATCCACTTCCCAACCAGCC | NM_214041 |
| R: CTCCCCATCACTCTCTGCCTTC | |||
| TNF-α | 151 | F: ATGGATGGGTGGATGAGAAA | X54001 |
| R: TGGAAACTGTTGGGGAGAAG | |||
| CLDN1 | 93 | F: CTGTGGATGTCCTGCGTGT | NM_001244539.1 |
| R: GGTTGCTTGCAAAGTGGTGTT | |||
| OCLN | 163 | F: GAGAGAGTGGACAGCCCCAT | NM_001163647 |
| R: TGCTGCTGTAATGAGGCTGC | |||
| ZO-1 | 200 | F: GATCCTGACCCGGTGTCTGA | XM_021098856 |
| R: TTGGTGGGTTTGGTGGGTTG | |||
| MUC2 | 90 | F: CCAGGTCGAGTACATCCTGC | XM_021082584.1 |
| R: GTGCTGACCATGGCCCC |
1GenBank accession reference number.