Table 1.
Details of primer pairs for the chicken LncFAM gene
| Gene | Primer, 5'-3' | Size, bp | Annealing temperature, °C | Accession number |
|---|---|---|---|---|
| LncFAM 97bp-insertion detection | F: TCTCCACCCATTTTTATGCTGC R: TGTTGCCAGAAATATGCTTGCT | 206/303 | 60 | NC_006088 |
| LncFAM polymorphic sites detection | F: CCCTACTCCCCTTTCCCTTTT R: GGAAACCCTGAGTCTGAAGATAGT | 1689 | 60 | NC_006088 |
| RT- LncFAM | F: TGTGCAAGGGATTGGCATCA R: CATTTCCAGAAAAGGGCCGTG | 157 | 60 | XR_003073445 |
| RT-FAM48A | F: TGAAAGTGCTCGTCAGAGACC R: AGACATGACAACGTCTCCTGC | 180 | 60 | NM_001006275 |
| RT-POSTN | F: CCACCACATGGTAAACAAGCG R: AACAGCAGTTAGGACACGGT | 202 | 60 | NM_001030541 |
| GAPDH | F: GAACATCATCCCAGCGTCCA R: CGGCAGGTCAGGTCAACAAC | 132 | 60 | NM_204305 |