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Incorporating a minimal fluorescent probe into LexA. a) A schematic of the multiple macromolecular interactions in which the transcriptional repressor LexA participates during repression (left) or activation (right) of the SOS response. b) Modeled incorporation of Acd (blue), a minimally-perturbing fluorescent amino acid, at position 161 in LexA. The active site residues Ser119 and Lys156 are shown in orange. The inset depicts a rotated close-up of the incorporated Acd residue.
