Fig. 4.

Neutrophils mediate growth of bone metastatic PCa in vivo. a Luciferase-expressing C42B cells were injected intratibially into SCID Beige mice. Representative images, quantitation of bioluminescence (left graph), and change in tumor bioluminescence compared to start of study (right graph) of C42B (n = 5/group) treated with isotype control or anti-Ly6G 1A8 antibody 3 days after inoculation. Arrow denotes start of neutrophil depletion. b Quantitation (graph on left) and representative IF images (right) of C42B (denoted by cytokeratin (green)) expression of phosphorylated Histone H3 (red) in bone. Nuclei are stained with DAPI (blue). Size bar = 20 μm. Asterisks denote statistical significance (**p < 0.01) c Trichrome quantitation of saline-injected (sham) and tumor-inoculated tibia of isotype vs. anti-Ly6G-treated mice in representative graph (left); representative images (left) show Trichrome stain of tumor tibia with magnified inset. Type I collagen/bone is stained blue. Size bar = 500 μm. d Bioluminescence of luciferase-expressing C42B cells. C42B-luc cells were injected intratibially into SCID Beige mice (n = 5/group) and neutrophil depletion (via anti-Ly6G (1A8)) or isotype control treatments were started 2 weeks post-tumor inoculation (at arrow), denoted as late depletion