Fig. 1.

ITCs inhibit replication in both cancer and normal cells to a similar extend and in ROS-independent manner, although cancer cells viability is lower than normal cells. Prostate cancer cells (PC-3 and their Rho0 derivatives) and normal dermal fibroblasts (HDFa) were treated for 3 h (a, c) or 24 h (b) with indicated concentrations of SFN, PEITC or vehicle (DMSO; 0). a, c Cells were cultured in the presence of 2 µCi/ml of methyl-³H-thymidine. Thymidine incorporation was determined by liquid scintillation. Radioactivity of controls was taken as 100%. The results are expressed as the mean ± SE of 3 independent experiments. b Cell viability was assessed by SRB method. Each point is mean ± SE of two experiments done in triplicate. Significantly different at p < 0.01 (*) compared with control or between cell lines by one-way ANOVA followed by Bonferroni’s multiple comparison test; n.s. non significant