Fig. 2.

ITCs induce DNA damage. Prostate cancer cells (PC-3) and normal dermal fibroblasts (HDFa) were treated with DMSO (C), PEITC (10 µM), SFN (40 µM) or etoposide (20 µM) for 3 h. Alkaline comet assay was performed as described in “Materials and methods”. Experiment was performed in at least two independent replicates. a Representative images for each condition are shown. Magnifications of selected regions are shown on the right panels. b Olive tail moment was calculated to assess DNA integrity. Significantly different at p < 0.01 (*) between cell lines by one-way ANOVA followed by Bonferroni’s multiple comparison test