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. 2019 May 23;59(4):1421–1432. doi: 10.1007/s00394-019-01995-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Inhibition of replication by ITCs is a rapid process, preceding genotoxic stress appearance, and occurs with the same kinetics in normal and cancer cells. a HDFa and PC-3 were treated with 40 µM SFN, 10 µM PEITC or equal amount of pure vehicle (DMSO) for indicated times and percentage of cells with p-H2A.X (γH2A.X) was determined by flow cytometry. b HDFa and PC-3 cells were treated as in a in the presence of 2 µCi/ml of methyl-³H-thymidine. Thymidine incorporation was determined by liquid scintillation. Radioactivity in control samples at 60 min was taken as 100%. The results are expressed as the mean ± SE of 2–5 independent experiments