Fig. 6.

Comparison of DHA-induced pyroptosis and cisplatin-induced apoptosis by SEM. BV-2 cells were treated for 4 h with vehicle (a, d), 200 μM DHA (b, e), or 200 μM cisplatin (c, f). a Vehicle-treated cells show intact cellular morphologies with structured nuclei and cytoplasmic protrusions. b DHA-treated cells exhibit loss of cytoplasmic and nuclear organization, loss of cell processes, and disruptions of the plasma membrane (black arrows). c Cisplatin-treated cells were again characterized by prominent membrane blebs (white arrows) while maintaining apparent cytoplasmic and nuclear organization. d At higher magnification, vehicle-treated cells have intact plasma membranes with cytoplasmic protrusions (black arrowheads) and EVs (white arrowheads). e DHA-treated cells are again characterized by nuclear envelope disruptions and a lack of discernable organelles. f The nuclear envelope and cytoplasmic organelles of cisplatin-treated cells remain largely intact. Note that organelles are more tightly packed and have decreased electron density compared to vehicle-treated cells. Scale bars apply all panels in each row