Table 3.
The molecular ion and MS/MS product ions in five saponin-rich fractions
| No | Identity | Molecular formula | JRe | ERe | EBa | WBa | SBa | [M-H]− (m/z) mass accuracy < 10−5 | MS/MS fragment ion (m/z) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Cladoloside B | C59H92O26 | − | − | + | + | − | 1215.5805 | 1171.8, 995.6, 863.6, 701.5, 555.4, 423.4 |
| 2 | Holotoxin A1 | C66H104O31 | + | + | + | + | + | 1391.6488 | 1347.6, 1171.6, 1153.6, 1039.5, 1009.5, 893.5, 701.4, 423.4 |
| 3 | Echinoside A | C54H87O26SNa | + | + | + | + | + | 1205.5031 | 1183.5, 1085.2, 909.3, 747.4 |
| 4 | Holothurin A | C54H85O27SNa | + | + | + | + | + | 1219.4823 | 1201.8, 1093.6, 1039.8, 893.6, 917.6, 785.5, 423.4 |
| 5 | Holothurin A1 | C54H87O27SNa | + | + | − | + | − | 1221.4980 | 1203.8, 1095.6, 1041.7, 895.6, 733.6, 787.5, 425.3 |
“−” not detected, “+” detected