Table 1.
Characterization of 20 polymorphic microsatellite loci in the white-bellied pangolin (Phataginus tricuspis) from Cameroon
| Locus | Primer sequences (5′→3′) | Repeat Motif | Na | Size range—bp | Ho | He | AR | PHW | Genbank Acc. No | Dye | Multiplex |
|---|---|---|---|---|---|---|---|---|---|---|---|
| PT_34432 |
F: AGATATGGCACCCCAATGTTTC R: AAGTGTATGCACCCCTTTGC |
(AC)21 | 9 | 107–141 | 0.667 | 0.608 | 8.498 | 0.950 | MT109130 | VIC | II |
| PT_276641 |
F: TGAGTCCTCCAGATCAGCAC R: GAACTCAGGGAAATGGTGGC |
(TGAG)9 | 5 | 182–198 | 0.542 | 0.549 | 4.75 | 0.397 | MT109129 | VIC | IV |
| PT_308752 |
F: CTGCAACGGGATTTCCCTAC R: TGTTTCCCCAGGGCTTAGAG |
(TTCC)16 | 8 | 144–208 | 0.625 | 0.784 | 7.749 | 0.391 | MT109128 | NED | II |
| PT_338821 |
F: AGTCAACGATGCAATAGGAAAC R: TGGCATCTTTCTAGGCGTGG |
(TG)22 | 9 | 232–254 | 0.571 | 0.836 | 9.000 | 0.000a | MT109127 | PET | III |
| PT_353755 |
F: GGTCACATGCTGAAGTGCTC R: TGCAATGAGGTAGGGACACG |
(TTA)9 | 8 | 206–242 | 0.75 | 0.76 | 7.847 | 0.021 | MT109126 | 6-FAM | IV |
| PT_378852 |
F: CCGCTGTCTTTCCAGAATGC R: GTCCCCACGGAGCTTACTAC |
(CCT)7 | 3 | 163–175 | 0.5 | 0.546 | 2.999 | 0.389 | MT109125 | 6-FAM | IV |
| PT_464918 |
F: TGGCAATACTGGTGTTTAGGAAC R: CTGTCATGGGCTAGTCACAC |
(AAT)10 | 8 | 110–131 | 0.667 | 0.755 | 7.847 | 0.428 | MT109124 | 6-FAM | II |
| PT_619913 |
F: AGCGGAAGGGTCACTAATGG R: TCTGTGCTGCTTCATAAAAGAGG |
(TGAA)8 | 7 | 202–226 | 0.5 | 0.637 | 6.737 | 0.728 | MT109123 | NED | III |
| PT_739516 |
F: ACATGTGGCATAGTCTGTTGG R: AGAGGCCTATGGGACAGTAAG |
(TTTG)8 | 5 | 226–246 | 0.417 | 0.545 | 4.750 | 0.691 | MT109122 | VIC | III |
| PT_796077 |
F: GGGACAATGCCAAAGGGTAG R: CAAACATTTGGGGCGAGTTG |
(TTTA)13 | 9 | 157–201 | 0.542 | 0.829 | 8.871 | 0.105 | MT109121 | VIC | I |
| PT_839522 |
F: CAGAGCTTGTACGCCATCAG R: CAGTGACAGTGTAATCCTGCG |
(AC)20 | 6 | 203–217 | 0.435 | 0.783 | 5.988 | 0.000a | MT109120 | PET | I |
| PT_1162028 |
F: CCTTTGTTATCCACCGACCC R: AGAACAGTGCCTTATGGAAGC |
(CATC)9 | 7 | 142–166 | 0.708 | 0.619 | 6.624 | 0.842 | MT109119 | 6-FAM | I |
| PT_1225378 |
F: GGGACTTGATAATAGGGGGTC R: AGCTGGGAATCTCAAACTGG |
(AATT)8 | 5 | 227–247 | 0.542 | 0.469 | 4.873 | 0.978 | MT109118 | 6-FAM | III |
| PT_1453906 |
F: GACCCCCATCTTTATGGAAGC R: AGTATGCCACTTGATGGATGG |
(ATCT)9 | 4 | 177–193 | 0.208 | 0.489 | 3.874 | 0.003 | MT109117 | 6-FAM | II |
| PT_1594892 |
F: AAAACGGAAGTGAAATTAAAGCC R: TGTTGTTCAAGGGCCAACTG |
(TG)12 | 6 | 202–218 | 0.458 | 0.624 | 5.999 | 0.012 | MT109116 | VIC | II |
| PT_1669238 |
F: ACCCACAGGATGTGCTACTC R: AGATTTCCCATCTACCCCGC |
(GT)14 | 8 | 178–204 | 0.667 | 0.715 | 7.623 | 0.780 | MT109115 | PET | II |
| PT_1753627 |
F: GTCCATCTACGCAACCCAAG R: GTTTAACCAGCTCCCACAATTTC |
(TG)16 | 7 | 234–252 | 0.75 | 0.782 | 6.848 | 0.797 | MT109114 | 6-FAM | I |
| PT_1849728 |
F: TCAAGGAACTTAGCTTCAGTTTG R: AGACTTTCGGTGTCATCCCC |
(TG)11 | 5 | 112–120 | 0.583 | 0.526 | 4.998 | 0.560 | MT109113 | 6-FAM | IV |
| PT_1973508 |
F: GGCTAATGTACAGTCTGATGTTCTC R: GGGCCCACGTGATTCTATGG |
(CA)23 | 12 | 221–249 | 0.833 | 0.827 | 11.36 | 0.962 | MT109111 | NED | I |
| PT_2019332 |
F: CAGTGTAACCAAAAAGAGTCTGC R: ACAGTTTTGGCACCTTCAATTTATC |
(ATCT)10 | 8 | 190–222 | 0.875 | 0.731 | 7.625 | 0.967 | MT109112 | PET | IV |
The number of individuals was 24 for all loci
Na number of alleles, Ho observed heterozygosity, He expected heterozygosity, AR allelic richness, and PHW P-values for tests of Hardy–Weinberg equilibrium deviation after Bonferroni correction
aLoci with significant departure from Hardy–Weinberg equilibrium (adjusted P-value = 0.0025)