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. 2020 Mar 29;11(4):369. doi: 10.3390/genes11040369

Figure 5.

Figure 5

Knockdown MTMR3 facilitates chicken SMSCs proliferation. (A) The knockdown efficiency of MTMR3 gene in SMSCs by three small interfering RNAs (siRNAs) were detected by qRT-PCR. (B,C) The protein expression level of MTMR3 after interference by MTMR3-siRNA1 was detected by Western blotting. (D) The mRNA levels of cell proliferation-related genes were detected by qRT-PCR in SMSCs after MTMR3 knockdown. (E) CCK-8 assays for SMSCs after MTMR3 knockdown. (F) The statistical results of cell cycle analysis for SMSCs after MTMR3 knockdown. (G) EdU staining after the transfection of MTMR3-siRNA1 in SMSCs. (H) Proliferation rates of chicken SMSCs following MTMR3 knockdown. Results are shown as mean ± SEM and the data are representative of at least three independent assays. Student’s t-test were used to compare expression levels among different groups. * p < 0.05; ** p < 0.01.