Figure 2.

Effect of liposomes on MCF-7 and MDA-MB231 cell viability determined by crystal violet assay. After treatment with different doses of liposomes (10, 20, 50, or 100 µL of liposomes per 1 mL of culture media) for the indicated time (0, 24, 48, 72, and 96 h), cells were fixed and stained by 0.1% crystal violet for 30 min. After solubilization, absorbance was measured by a Perkin Elmer multilabel plate reader. Data are represented by the mean ± SEM of three independent experiments and are indicated as statistically significant for * p < 0.05, ** p < 0.01, and *** p < 0.001 based on a Student’s t-test.