Table 1.
Average and standard deviation (sd) for percent sequence similarity to Sanger sequence, length of matching nucleotides, and number and percent of demultiplexed reads used for the final consensus sequence from 100R, 500R, or 5KR read subsets demultiplexed with MiniBar or qcat. Statistics were calculated across all tissue types and extraction method samples.
| Subset | Demultiplexer | Average % ID (sd) | Average Alignment Length (bp) (sd) | Average Number of Clustered Reads (sd) | Average % Clustered Reads (sd) |
|---|---|---|---|---|---|
| 100 reads per sample (100R) | MiniBar | 99.99 (0.05) | 421.05 (0.21) | 97.5 (5.8) | 97.50% (0.06) |
| qcat | 100 (0.00) | 420.5 (0.86) | 97.45 (6.01) | 97.45% (0.06) | |
| 500 reads per sample (500R) | MiniBar | 99.97 (0.11) | 421.09 (0.43) | 484.5 (35.77) | 96.90% (0.07) |
| qcat | 100 (0.00) | 420.82 (0.59) | 483.68 (38.32) | 96.73% (0.08) | |
| 5000 reads per sample (5KR) | MiniBar | 99.88 (0.24) | 421.18 (0.8) | 4411.14 (916.69) | 88.22% (0.18) |
| qcat | 99.95 (0.18) | 420.41 (0.85) | 4456.14 (939.87) | 89.12% (0.19) |