Figure 3.

Tryptophan (Trp) depletion reduces the fusion indexes in C2C12 and human skeletal muscle myoblasts (HSMMs). (a) Immunofluorescence images of C2C12 cells cultured in differentiation medium with or without Trp for 5 days and labeled with DyLight 488-conjugated anti-myosin heavy chain (green) and DAPI (blue). (b) Total fusion index represents the number of nuclei in multinucleated myotubes, divided by the total number of nuclei in a field, with a myotube defined as a cell with at least two nuclei. (c) Proliferation and (d) fusion index of HSMMs. * p < 0.05.