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. 2020 Apr 9;9(4):467. doi: 10.3390/foods9040467

Figure 2.

Figure 2

The real-time PCR curves, along with the corresponding melting curve analysis, obtained during the specificity study of the two-allele specific upstream primers with both DNA targets: Olea europaea var Sylvestris (wild-type of olive) (a) and Olea europaea L. var Europaea (cultivated olive) (b). Each specific primer strictly amplifies the fully complementary DNA sequence. Tm: melting temperature, RFU: Relative Fluorescence Units.