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. 2020 Apr 9;9(4):467. doi: 10.3390/foods9040467

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) The real-time PCR curves obtained for different % DNA content (0–50%) of Olea europaea var Sylvestris (wild-type olive) DNA in binary mixtures with Olea europaea L. var Europaea (cultivated olive) DNA. (b) The allelic ratios of the binary DNA mixtures calculated as the ratio of the fluorescence intensity obtained with the upstream primer specific to Olea europaea var Sylvestris target versus the sum of the fluorescence intensity obtained by both allele-specific primers for Olea europaea var Sylvestris and var Europaea targets. All allelic ratios were close to the value of 0.5, which corresponds to a heterozygote sample. RFU: Relative Fluorescence Units.