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. 2020 Apr 9;9(4):467. doi: 10.3390/foods9040467

Table 1.

The primers used in the allele-specific, real-time PCR, two species-specific upstream primers and a common downstream primer, along with their melting temperatures (Tm).

Primer Name 5′–3′ Oligonucleotide Sequence Melting Temperature *
Olea europaea var Sylvestris upstream primer TGTCAATTTTAATCACTACTGC 62 °C
Olea europaea L. Europaea upstream primer TGTCAATTTTAATCACTACTGT 61 °C
Common downstream primer CTAGTAACTAATCCTAACATGGAA 64 °C

* according to Eurofins Scientific (Brussels, Belgium).